Московский семинар по биоинформатике - 24 сентября 2012

Московский семинар по биоинформатике
Понедельник, 24 сентября 2012, 18.00
МГУ, Лаб. корпус Б (факультет биоинженерии и биоинформатики), к. 221.

Иоахим Мессинг
Университет Ратгерс

История секвенирования ДНК методом дробового ружья

Joachim Messing
Waksman Institute of Microbiology, Rutgers University

History of Shotgun DNA Sequencing

Recently, the Wharton School ranked DNA sequencing behind the Internet, Cell Phone, Email, and Laptop Computers the greatest innovation of our times. However, it is not so much by which method short strings of nucleotides are sequenced than the concept of parallelization of sequencing reactions that have revolutionized the sequencing of entire genomes. Parallelization of sequencing is based on universal primers and has been greatly accelerated by automation and computational capacity in recent years. This work began nearly 25 years ago with the construction of single-stranded phage (M13mp series) and high-copy plasmid (pUC series) vectors with their host strains (JM series) to today's next generation sequencing efforts.

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