Автор: Administrator
Московский семинар по биоинформатике
Понедельник, 3 декабря 2012, 18.00
МГУ, Лаб. корпус Б (факультет биоинженерии и биоинформатики), к. 221.
Василий Студитский
МГУ им М.В. Ломоносова и University of Medicine and Dentistry of New Jersey
Mechanisms of Pol II Transcription through Chromatin and Histone Chaperone FACT Action
Transcription of eukaryotic genes by RNA polymerase II (Pol II) is
typically accompanied by nucleosome survival and minimal exchange of
histones H3/H4 carrying epigenetic marks. In some cases, nucleosomes
present high, regulated barrier to transcribing Pol II. Recently we have
shown that efficient transcription through a nucleosome is coupled with
formation of an intermediate (a small intranucleosomal DNA loop
(O-loop) containing transcribing Pol II complex) that guarantees
nucleosome survival during transcription. Histone chaperone FACT affects
the efficiency of both processes (transcription through chromatin and
nucleosome survival), most likely affecting the efficiency of formation
of the O-loop. The mechanism of FACT action during transcription through
chromatin will be presented.
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