Московский семинар по биоинформатике - 3 декабря 2012

Московский семинар по биоинформатике

Понедельник, 3 декабря 2012, 18.00
МГУ, Лаб. корпус Б (факультет биоинженерии и биоинформатики), к. 221.

Василий Студитский
МГУ им М.В. Ломоносова и University of Medicine and Dentistry of New Jersey

Mechanisms of Pol II Transcription through Chromatin and Histone Chaperone FACT Action

Transcription of eukaryotic genes by RNA polymerase II (Pol II) is typically accompanied by nucleosome survival and minimal exchange of histones H3/H4 carrying epigenetic marks. In some cases, nucleosomes present high, regulated barrier to transcribing Pol II. Recently we have shown that efficient transcription through a nucleosome is coupled with formation of an intermediate (a small intranucleosomal DNA loop (O-loop) containing transcribing Pol II complex) that guarantees nucleosome survival during transcription. Histone chaperone FACT affects the efficiency of both processes (transcription through chromatin and nucleosome survival), most likely affecting the efficiency of formation of the O-loop. The mechanism of FACT action during transcription through chromatin will be presented.